Microfabrication Class 1

Powerpoint Class1

Readings.

 Soft Lithography

Monolithic Microfabricated Valves and Pumps by Multilayer Soft Lithography

Microfluidic Large-Scale Integration

Improving the Resolution of 3D-Printed Molds for Microfluidics by Iterative Casting-Shrinkage Cycles

Physics of Microfluidic Mechanics Class 2

Powerpoint Class2

Readings

Microfluidics: Fluid physics at the nanoliter scale

Life at Low Reynolds Number

Effects of pressure and temperature on the binding of RecA protein to single-stranded DNA

Inkjet Microfluidics and DNA Printing Class 3

Powerpoint Class3

Readings

Printing Multistrain Bacterial Patterns with a Piezoelectric Inkjet Printer

POSAM: a fast, flexible, open-source, inkjet oligonucleotide synthesizer and microarrayer

Error correction in gene synthesis technology

Parallel on-chip gene synthesis and application to optimization of protein expression

 

Microfluidic Cell Culture Class 4

Powerpoint Class 4

Readings Class 4

A sensing array of radically coupled genetic ‘biopixels

The single-cell chemostat: an agarose-based microfluidic device for high-throughput, single-cell studies of bacteria and bacterial communities

A microfluidic chemostat for experiments with bacteria and yeast cells

Robust growth of Escherichia coli

Bacterial Persistence as a Phenotypic Switch

Long-Term Monitoring of Bacteria Undergoing Programmed Population Control in a Microchemostat

Plant Microfluidics Class 5

Powerpoint Class 5

Readings Class 5

Microfluidic platforms for plant cell studies

A microfluidic device and computational platform for high-throughput live imaging of gene expression

A microfluidic platform for the investigation of elongation growth in pollen tubes

The RootChip: An Integrated Microfluidic Chip for Plat Science

An organ-on-a-chip approach for investigating root-environment interactions in heterogeneous conditions

Live imaging of root bacteria interactions in a microfluidics setup

Matyas will talk about our root chip microfluidics experiments.

Droplet Microfluidics Class 6

Powerpoint Class 6 Final

Readings Class 6

Droplet microfluidics for high-throughput biological assays

Droplet microfluidics

Millifluidic droplet analyzer for microbiology

Growing microbes in milli-fluidic droplets

Ultrahigh-throughput screening in drop-based microfluidics for directed evolution

Exploring sequence space in search of functional enzymes using microfluidic droplets

Microfluidics and Biochemistry Class 7

Powerpoint Class 7 Final

Readings Class 7

Megapixel digital PCR

An integrated microfluidic system for rapid screening of alpha-fetoprotein-specific aptamers

Room-temperature serial crystallography using a kinetically optimized microfluidic device for protein crystallization and on-chip X-ray diffraction.

 A vesicle bioreactor as a step toward an artificial cell assembly

Programmable on-chip DNA compartments as artificial cells

Single-cell barcoding and sequencing using droplet microfluidics 

Microfluidics and E. coli Class 8

Powerpoint Class8Final3

Readings Class 8

A wall of funnels concentrates swimming bacteria

Memory and Fitness Optimization of Bacteria under Fluctuating Environments

Memory and modularity in cell-fate decision making

Cell-Size Control and Homeostasis in Bacteria

Quantifying E. coli Proteome and Transcriptome with Single-Molecule Sensitivity in Single Cells

Microfluidics and Model Organisms Class 9

Powerpoint Class9Final2

Readings Class 9

Microfluidics for in vivo imaging of neuronal and behavioral activity in Caenorhabditis elegans

Microfluidic system for on-chip high-throughput whole-animal sorting and screening at subcellular resolution

Dynamics of Drosophila embryonic patterning network perturbed in space and time using microfluidics

Detection of Dynamic Spatiotemporal Response to Periodic Chemical Stimulation in a Xenopus Embryonic Tissue

Robust Spatial Sensing of Mating Pheromone Gradients by Yeast Cells

Microfluidics and Biotic Games Class 10

Powerpoint Class 10 Biotic Games

Readings Class 10′

Microfluidics Immunology Class 11

Powerpoint Class 11 Immunology

Readings Class 11

A microfluidic device for measuring cell migration towards substrate-bound and soluble chemokine gradients

An agarose-based microfluidic platform with a gradient buffer for 3D chemotaxis studies

A micro engraving method for rapid selection of single cells producing antigen-specific antibodies

Ultrasensitive microfluidic solid-phase ELISA using an actuatable microwell-patterned PDMS chip

Microfluidic Devices for Blood Fractionation

Microfluidic single-cell analysis for systems immunology

Microfluidics Odds and Ends Class 12

Powerpoint Class 12

Readings Class 12

Thin PDMS Films Using Long Spin Times or Tert-Butyl Alcohol as a Solvent

Microfluidic very large scale integration (mVLSI) with integrated micromechanical valves

Sonochemistry and sonoluminescence in microfluidics

An Integrated Fluorescence Detection System in Poly(dimethylsiloxane) for Microfluidic Applications

Small but Perfectly Formed? Successes, Challenges, and Opportunities for Microfluidics in the Chemical and Biological Sciences